The AmScope IN300TB inverted trinocular compound microscope has interchangeable pairs of 10x22mm and 20x10mm high-eyepoint super-widefield eyepieces, an under-mounted quadruple nosepiece with four DIN long working-distance plan objectives, top-mounted Brightfield 30W halogen illumination, a 0.3 NA Abbe condenser with iris diaphragm, and a double-layer mechanical stage with a stage stop to protect slides and objectives from damage. The anti-mold trinocular head has a Siedentopf binocular mount with 50 to 75mm interpupillary adjustment and a fixed 45-degree vertical inclination to reduce eye and neck strain. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, eliminating the need to refocus the image. Anti-mold coatings protect the microscope in high-humidity environments. Interchangeable pairs of WH10x22mm and WH20x10mm high-eyepoint super-widefield eyepieces provide improved focus over the entire field of view (FOV). High-eyepoint objectives ease viewing for viewers who wear glasses. Dioptric adjustment on both eyepieces accommodates individual eye-strength differences. The vertical trinocular port accepts a 23mm or C-Mount camera (camera sold separately). The under-mounted nosepiece has 4x, 10xPH (phase-contrast), 20xPH (phase-contrast), and 40x (spring) DIN long working-distance plan objectives that combine with the eyepieces to provide color correction of magnified images. Long working-distance plan objectives provide improved focus over the entire range of the viewing field and a longer working distance that is required when viewing specimens in dishes and large containers. Phase-contrast provides high contrast and visibility without the use of stains, allowing specimens to be observed in their natural state without being killed or fixed. An inverted microscope has its light source above the stage pointing downward and the objectives and turret below the stage pointing upward. It is most often used for observing organisms in a large container, such as a well plate or petri dish. It can also be used when specimens require manipulation, or in metallurgical applications.
Brightfield, phase-contrast, and fluorescence illumination is mounted above the stage and has transmitted 30W halogen illumination. Brightfield (BF) illumination allows the specimen to absorb light, resulting in a dark image on a light background. The phase-contrast element aligns the light source with the phase-contrast objectives to optimize high contrast and visibility without the use of stains, allowing specimens to be observed in their natural state without being killed or fixed. Halogen illumination provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. Often used in biological and metallurgical research, fluorescence microscopy uses a xenon or mercury lamp to create ultraviolet light and excitation filters to filter unwanted wavelengths, allowing users to see details undetectable to other light sources. The movable 0.3 NA Abbe condenser can be adjusted to control the distance of the light from the stage, and the iris diaphragm has an opening that focuses and controls the amount of light illuminating the specimen from fully open to fully closed. The double-layer mechanical stage with 1mm stage divisions and 0.1mm vernier resolution locks the slide into place and provides precise slide manipulation along the X- and Y-axis to allow coordinates to be recorded, enabling the viewer to return to a specific location on the slide. The oversize stage is 9.53 x 6.77 inches (242 x 172mm) and has a traveling range of 4.53 x 2.59 inches (115 x 75mm) (W x D, where W is width, the horizontal distance from left to right; D is depth, the horizontal distance from front to back). Nested coaxial coarse and fine focus has tension-adjustable coarse focusing and an adjustable lock ring stopper that limits stage range to protect slides and objectives from damage. All-metal mechanical parts, solid-metal frame construction, and a stain-resistant enamel finish provide durability.
SpecificationsHeadSiedentopf trinocularMagnification range40x-800xTrinocular port23mm or C-MountEyepieces (30mm)WH10x20mm and WH20x10mm high-eyepoint super-widefield planObjectives (20mm)4x, 10xPH, 20xPH, and 40x DIN long working-distance plan objectivesStageDouble-layer mechanicalCondenserMovable 0.3 NA Abbe with iris diaphragmLight sourceHalogen with rheostat, 12V/30WIllumination typeBrightfield, phase-contrastPower110V, UL-listed
Microscopes are instruments used to enhance the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, rather than looking through a lens. Microscopes can have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers to the size of an image. Resolution, also known as resolvant power, refers to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can control magnification through a fixed focus, or through a range of adjustments. They can also utilize LED, fluorescent, and mirror light sources to help control viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and in the medical, forensic science, and veterinary industries.
United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.
What's in the Box?AmScope IN300TB microscope
WH10x high-eyepoint super-widefield eyepieces, 30mm, one pair
WH20x high-eyepoint super-widefield eyepieces, 30mm, one pair
4x DIN long working-distance plan objective, 20mm
10x DIN long working-distance phase-contrast plan objective, 20mm
20xPH DIN long working-distance phase-contrast plan objective, 20mm
40xS DIN long working-distance plan objective, 20mm
(3) Color filters; yellow, blue, and green
Reflected illuminations system with top light housing and electrical cord
Episcopic light box and tube
Power cord
Dust cover
Instructions