Background Information:
LB stands for Luria Broth, a nutritionally rich medium, which is one of the most common media used in laboratories worldwide. LB medium is recommended as safe for student use.
Kit Content:
1) 10 x Pre-poured sterile LB-agar plates (100 x 15 mm Petri Dish)
2) 10 x 6-inch sterile cotton swabs (2 per pouch)
3) Detailed Instruction Sheet
4) Free Science Fair Project E-Book
Storage Condition:
LB agar plates should be placed in a refrigerator upon arrival. LB-agar plates stored under such conditions have a minimum shelf life of 3 months.
Brief Instruction:
1) Take out one sterile cotton swab. Gently wipe a test area with the cotton swab to pick up bacteria. Here are some of the popular samples of the test objects: human or pet teeth, hands, phone, TV remote, restaurant menu, sink, floor, fruit, vegetable, etc.
2) Take out one of the agar plates (No warm-up is needed). Open the agar plate and gently streak the agar plate with the cotton swab to transfer the bacteria onto the surface of the agar plate.
3) Cover the agar plate and place it in an upside-down position in an incubator with a temperature between 85 and 100 oF. A desk lamp or a portable heater can be used as a heat source.
4) Watch the appearance of small bacterial colonies on the surface of the agar plates after 12-48 hr of incubation.